Brain disease model systems?

To better understand the complex state of the brain pathology, we seek to recapitulate the brain microenvironment that can address changes in physiological activity, cell-cell network, and transcriptome specific to a neurodegenerative disease, traumatic brain injury (TBI), brain cancer, or neuroinflammation.

1. Experimental brain model systems (pathological,diagnostic, regenerative)

    a. Brain cancerproject: What do glioma cells urge neurons to do for their growth?

        - cortical organoids, tissue clearing, synapticnetwork/activity, field recording, single cell analysis

    b. Braininfection project: How do bacterial lipopeptides invade into brain?

        - 3D printed BBB-on-a-chip, stage of activated microglia/astrocytes,real time imaging. 

    c. Hydrocephalus project: How do hemorrhage-induced iron contents induceventricular enlargement? Can we reverse it?

        - choroid plexus organoids, stereotactic surgeries of mice, biomarkerassay, MRI scan

2. Development of new parameters for detection of therapeuticsignatures specific to brain disease

    a. Brain cancer project: Quantitativeculture model to evaluate the efficacy of CAR-T cells 

        - 3D neural tissue culturematrix, physical and mathematical model

    b. Nerve regenerationproject: Electric field and neural differentiation 

       - E-stim platform, singlecell analysis, 

       - Image processing (e.g., FFT, Markov chain) to correlate morphological abnomality with transcriptomic changes